A DOT urine collection is more structured than most people expect. Every step exists to keep the result accurate and legally defensible.
The Federal CCF
Every DOT collection uses a Federal Custody and Control Form (CCF). It records the donor, the reason for the test, and the chain of custody from collection to lab to MRO. A mistake on the CCF can invalidate a test, which is why trained collectors matter.
The Split Specimen
The donor's sample is split into a primary (Bottle A) and a split (Bottle B). If the primary tests positive and the donor disputes it, Bottle B can be tested at a second lab. This protects the driver.
Key Steps
- Photo ID and reason for test recorded on the CCF
- Temperature checked within four minutes to confirm a fresh sample
- Sample split, sealed, and initialed by the donor
- Chain of custody signed at every handoff
Frequently Asked Questions
What is the split specimen for? It lets a driver request a second lab test of the Bottle B sample if they dispute a positive result on Bottle A.
Why is temperature checked? An out-of-range temperature can indicate a substituted or tampered sample and triggers a new, observed collection.
What if the CCF has an error? Some errors can be corrected with a memo; others invalidate the test. Trained collectors keep this from happening.
Let Test Pro Handle It
Test Pro LLC in Kent, WA helps owner-operators, motor carriers and employers stay compliant across Washington and beyond — drug and alcohol testing, random pools, IFTA, filings and driver files. Call (206) 518-2097 or book an appointment. Questions first? Contact us.